Sullivan, Westerneng, Haynes, Bennett & Coleman (1995)

Macroscopic morphology

Colonies on Sabouraud dextrose agar at 25°C are white to cream, soft, and smooth to wrinkled. This isolate has poor to no growth at 42°C. Colonies grown on Chromager are dark green as opposed to the light blue-green exhibited by C. albicans.

Microscopic morphology

On cornmeal following 72 hours incubation at 25°C, abundant branched pseudohyphae and true hyphae with blastoconidia are present. Many strains produce an abundance of chlamydoconidia resulting in excess of 25-30 being observed in each microscopic field. Chlamydoconidial arrangement includes single, pairs, chains, and clusters.

Special Notes

This isolate is germ tube positive which accounts for its historic miss-identification as C. albicans. Initially thought to be associated only with HIV disease, several reports have been published since its description implicating it in non-HIV associated infection as well [381], [704], [1543]. Molecular analysis show that C. dubliniensis is distinct from C. albicans by 13-15 nucleotides in the ribosomal RNA gene sequences [2191]. Early reports purported that C. dubliniensis was responsible for fluconazole-resistant thrush but susceptibility studies reveal that it’s categorical distribution is similar to C. albicans with isolates ranging from susceptible to resistant.

FTL* in vitro susceptibility data

<0.03 µg/ml=1 <0.03 µg/ml=11 <0.125 µg/ml=3 <0.125 µg/ml=9 <0.015 µg/ml=9 <0.015 µg/ml=9 <0.03 µg/ml=4
0.06 µg/ml=4 0.06 µg/ml=5 0.25 µg/ml=9 0.03 µg/ml=3 0.03 µg/ml=1
0.125 µg/ml=6 0.125 µg/ml=2 0.5 µg/ml=2 0.06 µg/ml=1 1.0 µg/ml=1
0.25 µg/ml=1 0.25 µg/ml=2 2.0 µg/ml=1 0.5 µg/ml=1 >8 µg/ml=1
1.0 µg/ml=1 4.0 µg/ml=1
>64 µg/ml=2


Drug/N AMB/13 CAS/20 5FC/3 FLU/24 ITRA/14 VORI/12 KETO/4
MIC Range <0.03-1.0 <0.03-0.25 <0.125 <0.125-64 <0.015-0.5 <0.015->8 <0.03
MIC50 0.125 <0.03 0.25 <0.015 <0.015
MIC90 0.25 0.125 4.0 0.06 1.0

* Fungus Testing Laboratory unpublished data (NCCLS M27-A2)