Sullivan, Westerneng, Haynes, Bennett & Coleman (1995)
Colonies on Sabouraud dextrose agar at 25°C are white to cream, soft, and smooth to wrinkled. This isolate has poor to no growth at 42°C. Colonies grown on Chromager are dark green as opposed to the light blue-green exhibited by C. albicans.
On cornmeal following 72 hours incubation at 25°C, abundant branched pseudohyphae and true hyphae with blastoconidia are present. Many strains produce an abundance of chlamydoconidia resulting in excess of 25-30 being observed in each microscopic field. Chlamydoconidial arrangement includes single, pairs, chains, and clusters.
This isolate is germ tube positive which accounts for its historic miss-identification as C. albicans. Initially thought to be associated only with HIV disease, several reports have been published since its description implicating it in non-HIV associated infection as well , , . Molecular analysis show that C. dubliniensis is distinct from C. albicans by 13-15 nucleotides in the ribosomal RNA gene sequences . Early reports purported that C. dubliniensis was responsible for fluconazole-resistant thrush but susceptibility studies reveal that it’s categorical distribution is similar to C. albicans with isolates ranging from susceptible to resistant.
FTL* in vitro susceptibility data
|<0.03 µg/ml=1||<0.03 µg/ml=11||<0.125 µg/ml=3||<0.125 µg/ml=9||<0.015 µg/ml=9||<0.015 µg/ml=9||<0.03 µg/ml=4|
|0.06 µg/ml=4||0.06 µg/ml=5||0.25 µg/ml=9||0.03 µg/ml=3||0.03 µg/ml=1|
|0.125 µg/ml=6||0.125 µg/ml=2||0.5 µg/ml=2||0.06 µg/ml=1||1.0 µg/ml=1|
|0.25 µg/ml=1||0.25 µg/ml=2||2.0 µg/ml=1||0.5 µg/ml=1||>8 µg/ml=1|
|1.0 µg/ml=1||4.0 µg/ml=1|
* Fungus Testing Laboratory unpublished data (NCCLS M27-A2)