van Uden & do Carmo-Sousa (1959)

Teleomorph:

Clavispora lusitaniae Rodrigues de Miranda (1979)

Macroscopic morphology

Colonies on Sabouraud dextrose agar at 25°C are white to cream, glistening, and smooth but may be rugose. Colonies may be fringed with pseudohyphae. This isolate grows both at 42°C and on media containing cycloheximide.

Microscopic morphology

On cornmeal following 72 hours incubation at 25°C, abundant branched pseudohyphae may be present. Some strains have rudimentary to no pseudohyphae.

Special notes

Candida lusitaniae is known for amphotericin B resistance. The first report of fatal infection occurred in a neutropenic patient where the initial isolate was susceptible to amphotericin B but subsequent isolates had developed amphotericin B resistance [1712]. It is well-documented that this species is able to develop resistance, but some strains display resistance prior to amphotericin B therapy [1524]. Although amphotericin B resistance is a concern, reports exist where amphotericin B has been used to successfully treat fungemia in severely immunocompromised patients [259].

FTL* in vitro susceptibility data

AMB CAS 5FC FLU ITRA VORI KETO
0.06 µg/ml=2 <0.03 µg/ml=6 <0.125 µg/ml=41 <0.125 µg/ml=7 <0.015 µg/ml=13 <0.015 µg/ml=27 <0.03 µg/ml=8
0.125 µg/ml=35 0.06 µg/ml=33 0.25 µg/ml=1 0.25 µg/ml=24 0.03 µg/ml=23 0.03 µg/ml=16 0.06 µg/ml=6
0.25 µg/ml=72 0.125 µg/ml=50 4.0 µg/ml=1 0.5 µg/ml=57 0.06 µg/ml=24 0.06 µg/ml=5 0.125 µg/ml=4
0.5 µg/ml=14 0.25 µg/ml=18 64 µg/ml=1 1.0 µg/ml=42 0.125 µg/ml=12 0.125 µg/ml=5 0.25 µg/ml=1
1.0 µg/ml=5 0.5 µg/ml=3 >64 µg/ml=2 2.0 µg/ml=9 0.25 µg/ml=8 0.25 µg/ml=3
2.0 µg/ml=1 1.0 µg/ml=4 16 µg/ml=3 0.5 µg/ml=1 0.5 µg/ml=3
2.0 µg/ml=1 32 µg/ml=4 4.0 µg/ml=1 2.0 µg/ml=1
64 µg/ml=6 4.0 µg/ml=1
>64 µg/ml=1 8.0 µg/ml=1

 

Drug/N AMB/129 CAS/115 5FC/46 FLU/153 ITRA/82 VORI/62 KETO/19
MIC Range 0.06-2.0 <0.03-2.0 <0.125->64 <0.125->64 <0.015-4.0 <0.015-8 <0.03-0.25
MIC50 0.25 0.125 <0.125 0.5 0.06 0.03 0.06
MIC90 0.5 0.25 0.25 2.0 0.25 0.25 0.125

* Fungus Testing Laboratory unpublished data (NCCLS M27-A2)
 

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